Figure 5

ZD6474 inhibits migration in breast cancer cells in combination with UV-B by inhibiting VEGF signaling pathway. Scratching across a cell monolayer on a 6-well culture plate created a wound and the width of the wound was recorded prior to treatment with ZD6474 and/or UV-B and after indicated h post-treatment. Photomicrograph of ZD6474 and/or UV-B treated (A) MCF-7 and (B) MDA-MB-468 cells after 48 h and 24 h post-treatment respectively. Scale bar, 100 μM. Bars, S.E., three random widths along the wound at indicated h post-treatment of (C) MCF-7 and (D) MDA-MJ B-468 cells. P-values were calculated by using un-paired t-test of the same treatment groups (prior and after stipulated h post-treatment). Breast cancer cells were treated with ZD6474 and/or UV-B and incubated in serum-free CM for 48 h. VEGF level of (E) MCF-7 and (F) MDA-MB-468 was determined by ELISA. All data were derived from a minimum of three independent experiments using different cell preparations. *P < 0.05, comparing levels of untreated control with treated cells.