Figure 5

TIAM1 is a direct target of miR-29c and involved in NPC cell growth and invasion. (A) Wild-type or mutant miR-451 target sequences of MIF 3′ UTR. (B) Relative luciferase activity of SUNE-1 and CNE-2 cells after co-transfection with wild type (Wt) or mutant (Mt) MIF 3′ UTR reporter genes and miR-29c mimics or control. (C, D) Quantification of MIF mRNA (C) and protein (D) expression after transfection with miR-451 mimics or control. (E, F) Colony formation (E) and invasive ability (F) after transfection with siMIF or control. Data is presented as the mean ± SD. *, P < 0.01 compared with control using the Student’s t-test.