The Notch pathway is analogously orchestrated throughout CRC progression. (A) Representative confocal microscopy images of MUC2 (green) in human cultures treated with 10 μM dibenzazepine (DBZ) show differentiation into the goblet cell-lineage as depicted by increased MUC2 expression (B) The number of goblet cells was quantified by counting the number of MUC2+ cells from 10 random fields at 63× magnification. Values shown are the percentages of MUC2+ cells relative to the total number of cells. (C) The increased MUC2 expression was further measured with qRT-PCR. MUC2 mRNA levels were normalized to GAPDH mRNA and expressed as fold induction compared with DMSO control. The graph represents the mean ± SD of three different experiments. (D) DBZ treatment results in reduced clonogenicity. The results depicted are representative from three independent experiments from human healthy colon organoid (N=2), adenoma (N=3) and adenocarcinoma (N=3). Scale bar: 50 μm. * p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001 (t-test).