Immunohistochemical (IHC) staining for Oct4, Nestin, and NeuN in primary NSCLC. Shown are representative images. (B, E, G) Negative controls. Positive cells are stained brown. (A) Seminoma was used as positive control for Oct4. (C) shows nuclear staining in cancer cells for Oct4 and (D) shows cytoplasmic expression of Oct4. (F-H) Cancer cells expressing Nestin or NeuN expression. (aa) Magnified image of cells expressing NeuN. (I-J) Chromogranin and synaptophysin were not expressed in the NSCLC shown above.