Inhibition of BMP signaling decreases Id1 and Id3 expression and inhibits cell growth of Nestin/GFP, Oct4/GFP, and GFP (−) cells. (A) Western blot analysis of the different cell populations isolated from H1299 cells treated with 1 μM DMH2 for 48 hours. DMH2 decreases pSmad 1/5 and its transcriptional target Id1 and Id3 in all cell populations. (B) Cells were treated with 1 μM DMH2 for 7 days and live cells counted. Data represents the mean of 3 independent experiments depicted as percent of vehicle control. (C) Oct4/GFP and Nestin/GFP cells were treated with 1 μM DMH2 for 48 hours and the percent cells that take up Ethidium bromide counted. Data represents the mean of 4 independent experiments shown as percent of vehicle control. Significant cell death occurred only in the Nestin/GFP cells. (D) Quantitative RT-PCR for nestin of Oct4/GFP and Nestin/GFP cells treated with 1 μM DMH2 for 48 hours. Data represents the mean of at least 3 independent experiments. Inhibition of BMP signaling induced nestin expression in Oct4/GFP cells but not Nestin/GFP cells.