Figure 6From: Small molecule antagonist of the bone morphogenetic protein type I receptors suppresses growth and expression of Id1 and Id3 in lung cancer cells expressing Oct4 or nestinInhibition of BMP signaling decreases Id1 and Id3 expression and inhibits cell growth of Nestin/GFP, Oct4/GFP, and GFP (−) cells. (A) Western blot analysis of the different cell populations isolated from H1299 cells treated with 1 μM DMH2 for 48 hours. DMH2 decreases pSmad 1/5 and its transcriptional target Id1 and Id3 in all cell populations. (B) Cells were treated with 1 μM DMH2 for 7 days and live cells counted. Data represents the mean of 3 independent experiments depicted as percent of vehicle control. (C) Oct4/GFP and Nestin/GFP cells were treated with 1 μM DMH2 for 48 hours and the percent cells that take up Ethidium bromide counted. Data represents the mean of 4 independent experiments shown as percent of vehicle control. Significant cell death occurred only in the Nestin/GFP cells. (D) Quantitative RT-PCR for nestin of Oct4/GFP and Nestin/GFP cells treated with 1 μM DMH2 for 48 hours. Data represents the mean of at least 3 independent experiments. Inhibition of BMP signaling induced nestin expression in Oct4/GFP cells but not Nestin/GFP cells.Back to article page