ucRNA expression in prostate tumors and regulation of ucRNAs by epigenetic drugs and the androgen receptor ligand, R1881, using NanoString nCounter-based quantification. (A) Up-regulation of uc.363 + A and uc.477 and down-regulation of uc.454 + A in primary prostate tumors versus the adjacent non-cancerous tissues. Expression was measured using the NanoString nCounter system, as described in the methods. The uc.363 + A transcript was found to be most notably up-regulated in stage 3 tumors and expression between stage 2 and stage 3 tumors was significantly different (consistent with the array data). (B) Nanostring-based analysis of uc.283 + A expression in LNCaP cells treated with epigenetic drugs. Expression of uc.283 + A was significantly up-regulated after treatment with 5-AzaC and TSA (P < 0.05 with one-way ANOVA and Tukey’s posthoc test), with a significant trend from untreated to mono treatment to combination treatment (P < 0.05). (C) Nanostring-based analysis of uc.287+ and uc.283 + A expression in LNCaP cells treated with R1881. Three independent experiments were performed with 10 nM R1881. Shown are the expression changes from untreated to treated for the experiments (mean of n = 3 per individual experiment; P < 0.05; paired t-test).