Figure 2
From: Transcription signatures encoded by ultraconserved genomic regions in human prostate cancer
ucRNA expression in prostate tumors and regulation of ucRNAs by epigenetic drugs and the androgen receptor ligand, R1881, using NanoString nCounter-based quantification. (A) Up-regulation of uc.363 + A and uc.477 and down-regulation of uc.454 + A in primary prostate tumors versus the adjacent non-cancerous tissues. Expression was measured using the NanoString nCounter system, as described in the methods. The uc.363 + A transcript was found to be most notably up-regulated in stage 3 tumors and expression between stage 2 and stage 3 tumors was significantly different (consistent with the array data). (B) Nanostring-based analysis of uc.283 + A expression in LNCaP cells treated with epigenetic drugs. Expression of uc.283 + A was significantly up-regulated after treatment with 5-AzaC and TSA (P < 0.05 with one-way ANOVA and Tukey’s posthoc test), with a significant trend from untreated to mono treatment to combination treatment (P < 0.05). (C) Nanostring-based analysis of uc.287+ and uc.283 + A expression in LNCaP cells treated with R1881. Three independent experiments were performed with 10 nM R1881. Shown are the expression changes from untreated to treated for the experiments (mean of n = 3 per individual experiment; P < 0.05; paired t-test).