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Figure 3 | Molecular Cancer

Figure 3

From: The long non-coding RNA ERIC is regulated by E2F and modulates the cellular response to DNA damage

Figure 3

ERIC is cell cycle regulated. WI38 cells were growth arrested by serum deprivation (48 hours in medium with 0.1% serum, S = starvation) and then allowed to resume growth by serum addition (to a final concentration of 15%) for the times indicated. A) RNA was extracted and RNA levels of ERIC were determined by Real-time RT-PCR and normalized to GAPDH levels. B) Cell cycle distribution was determined using FACS analysis. Percentage of cells in G1, S and G2/M are indicated. C) WI38 cells were transfected with either a nonspecific siRNA (siNS) or an siRNA directed against E2F1 (siE2F1). Then cells were growth arrested by serum deprivation (48 hours in medium with 0.1% serum, S = starvation) and then allowed to resume growth by serum addition (to a final concentration of 15%) for the times indicated. Left panel-RNA was extracted and RNA levels of ERIC were determined by Real-time RT-PCR and normalized to GAPDH levels. Right panel-Proteins were extracted from the cells, and western blot analysis performed using antibodies directed against E2F1 and actin.

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