Phospho-kinase array in control and irradiated UT-SCC24A cells. Example of phospho-kinase array in untreated UT-SCC24A cells and 1 h after 4 Gy irradiation. Cell lysates were incubated with membranes containing capture antibodies (spotted in duplicate) against 46 kinase phosphorylation sites. Thereafter, the membranes were incubated with a cocktail of biotinylated detection antibodies and streptavidin-HRP. Proteins were detected using chemiluminescence and expression levels were quantified by measuring the integrated optical density (IOD) of each spot.