Figure 3

Western blot analysis of phospho-kinases after treatment with inhibitor and/or radiotherapy. UT-SCC5, 24A and 40 cells were incubated with the inhibitor overnight (16 h), irradiated with 4 Gy and harvested 4 h after radiotherapy treatment or 20 h after kinase treatment. A) AKT inhibitor: MK-2206 (2 μM), B) SFK inhibitor: dasatinib (100 nM), C) MEK1/2 inhibitor: U0126 (10 μM), D) p38 inhibitor: SB203580 (10 μM), E) STAT5 inhibitor: 573108 (100 μM), F) STAT6 inhibitor: leflunomide (100 μM). α-tubulin was used as loading control.