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Figure 1 | Molecular Cancer

Figure 1

From: Omega 3 fatty acids chemosensitize multidrug resistant colon cancer cells by down-regulating cholesterol synthesis and altering detergent resistant membranes composition

Figure 1

Effects of ω 6PUFAs and ω 3PUFAs on cholesterol synthesis and cells viability in colon cancer cells. HT29 and HT29-dx cells were incubated for 24 h in the absence (CTRL) or in the presence of various concentrations (25, 50, 100, 200 μM) of arachidonic acid (AA), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and subjected to the following investigations. (A) Cells were grown in a medium containing [3H]acetate, then the de novo synthesis of cholesterol was measured in duplicate as described in Methods. Data are presented as means ± SD (n = 4). Versus CTRL HT29 cells: * p < 0.05; versus CTRL HT29-dx cells: ° p < 0.05. (B) Cells were stained with Neutral Red solution and the absorbance of viable cells was measured in triplicate spectrophotometrically. Data are presented as means ± SD (n = 4). Versus respective CTRL: * p < 0.05.

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