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Figure 2 | Molecular Cancer

Figure 2

From: Omega 3 fatty acids chemosensitize multidrug resistant colon cancer cells by down-regulating cholesterol synthesis and altering detergent resistant membranes composition

Figure 2

ω 3PUFAs decrease activity and expression of HMGCoAR in chemoresistant colon cancer cells. HT29 and HT29-dx cells were incubated for 24 h in the absence (CTRL) or in the presence of 50 μM arachidonic acid (AA), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA). Simvastatin (1 μM for 24 h, SIM) was chosen as HMGCoAR inhibitor. Cells were lysed and centrifuged to collect the microsomal fraction, on which the following investigations were performed. (A) HMGCoAR enzymatic activity was measured in duplicate (see Methods). Data are presented as means ± SD (n = 3). Versus CTRL HT29: * p < 0.02; versus CTRL HT29-dx: ° p < 0.05. (B) Western blotting experiments were performed using an anti-HMGCoAR antibody; an anti-calreticulin (CRT) antibody was used as a control of equal protein loading. The figure is representative of three experiments with similar results. The band density ratio between HMGCoAR and CRT was expressed as arbitrary units. Versus CTRL HT29: * p < 0.05; versus CTRL HT29-dx: ° p < 0.05.

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