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Figure 7 | Molecular Cancer

Figure 7

From: Omega 3 fatty acids chemosensitize multidrug resistant colon cancer cells by down-regulating cholesterol synthesis and altering detergent resistant membranes composition

Figure 7

ω 3PUFAs restore the anti-proliferative effects of irinotecan in chemoresistant colon cancer cells. HT29 and HT29-dx cells were incubated for 48 h in the absence (CTRL) or presence of 50 μM docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA). (A) Irinotecan (CPT11) was added at increasing concentrations in the last 24 h. Cells were stained with Neutral Red solution and the absorbance of viable cells was measured in triplicate spectrophotometrically. Data are presented as means ± SD (n = 4). Versus respective CTRL: * p < 0.001; versus CPT11 alone: ° p < 0.005. (B) 10,000 HT29 and HT29-dx cells were seeded in 96-wells plates. After 8 h, the medium was replaced with fresh medium (CTRL), medium containing 50 μM docosahexaenoic acid (DHA), 50 μM eicosapentaenoic acid (EPA) or 1 μM irinotecan (CPT11), alone or in combination. Cell proliferation was monitored at 12, 24, 48, 72, 96 h by crystal violet staining. Measurements were performed in triplicate and data are presented as means ± SD (n = 4). Versus respective CTRL: * p < 0.01; versus CPT11 alone: ° p < 0.01.

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