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Table 5 Median and mean fluorescence intensity (MFI) of surface Pgp, MRP1 and BCRP in HT29 and HT29-dx cells

From: Omega 3 fatty acids chemosensitize multidrug resistant colon cancer cells by down-regulating cholesterol synthesis and altering detergent resistant membranes composition

 

Pgp

Pgp

MRP1

MRP1

BCRP

BCRP

Sample

Median

MFI

Median

MFI

Median

MFI

HT29 CTRL

8.24 ± 0.59

7.21 ± 0.11

5.15 ± 0.43

4.61 ± 0.41

5.37 ± 0.25

4.71 ± 0.26

HT29 AA

10.02 ± 2.25

8.58 ± 1.11

6.35 ± 0.54

5.52 ± 0.36

6.80 ± 0.21

5.62 ± 0.37

HT29 DHA

14.93 ± 2.02

8.74 ± 0.92

5.49 ± 0.29

5.25 ± 0.44

7.12 ± 0.47

6.49 ± 0.36

HT29 EPA

11.73 ± 1.01

10.09 ± 0.98

6.00 ± 0.47

6.55 ± 0.34

6.15 ± 0.29

5.57 ± 0.19

HT29-dx CTRL

34.84 ± 3.66*

31.06 ± 3.52*

34.01 ± 2.15*

32.20 ± 1.08*

5.15 ± 0.23

4.96 ± 0.23

HT29-dx AA

48.83 ± 4.72*

43.32 ± 4.98*

44.04 ± 5.53*

41.05 ± 5.11*

7.74 ± 0.83

7.23 ± 0.41

HT29-dx DHA

6.41 ± 0.52°

5.83 ± 0.41°

3.38 ± 0.41°

3.16 ± 0.37°

7.69 ± 0.79

7.23 ± 0.59

HT29-dx EPA

8.26 ± 0.51°

7.50 ± 0.21°

3.31 ± 0.24°

2.89 ± 0.13°

8.17 ± 0.89

7.64 ± 0.48

  1. HT29 and HT29-dx cells were left untreated (CTRL) or treated with AA, DHA, EPA (50 μM for 48 h).
  2. Data were calculated with the Cell Quest software and are presented as mean ± SD (n = 3). Versus CTRL HT29: *p < 0.002; versus CTRL HT29-dx: ° p < 0.001.