Figure 5

Ets-1 redox regulation involves changes in HIF-1 and GPX-2 protein levels. A) The protein expression of redox balance-related factors was examined by Western blot. B) Both xCT and GPX-1 protein are increased in correlation with Ets-1 overexpression, while HIF-1α and GPX-2 showed a trend to decreased with higher levels of Ets-1. C) In 2008 cells, HIF-1α and GPX-2 were decreased in response to PPG treatment, while GPX-1 was increased with both PPG and SAS. D) In 2008-Ets1 cells, decreased protein expression was observed for HIF-1α and GPX-2, while SAS also decreased GPX-2 protein levels. E) Similarly, tetracycline-induced 2008-Ets1 cells displayed decreased protein expression of HIF-1α and GPX-2 following PPG and SAS treatment.