Figure 1
From: Asymmetric segregation of template DNA strands in basal-like human breast cancer cell lines
Human breast cancer cell lines asymmetrically segregate their template DNA strands. (A) Schematic representation of the BrdU labeling assay. Chromosomes were labeled with BrdU (shown as green lines). BrdU was then removed from the media and cells were cultured for two cell divisions. Newly synthesized DNA is shown as blue lines. Due to semi-conservative DNA replication, asymmetric segregation of template DNA is apparent at the 2nd anaphase. (B) Representative images of a HCC1143 cell in anaphase with random segregation of its template DNA to both sets of chromosomes. (C) Representative images of an anaphase HCC1143 cell with template DNA segregated exclusively to one set of chromosomes, on the right bottom. Blue: DAPI-labeled DNA. Green: BrdU-labeled DNA.