Figure 3
From: Asymmetric segregation of template DNA strands in basal-like human breast cancer cell lines
Asymmetric segregation of template DNA is correlated with CD44+/CD24-/loexpression. (A) Breast cancer cell lines were divided into two groups according to the percentage of BrdU asymmetric division observed, high asymmetry (mean, ≥ 0.5%) and low asymmetry (mean, < 0.5%), then plotted against the percentage of cells that were CD44+/CD24-/lo. Cell lines with high asymmetry had a higher percentage of cells that were CD44+/CD24-/lo as compared to cell lines with low asymmetry (P = 0.004, Mann–Whitney test). Samples were analyzed in triplicates and in at least two independent experiments. (B) Fold-change in frequency of asymmetric segregation of template DNA in HCC1143 cells, normalized to parental-unsorted population (unsorted), are shown for cells that were sorted without gating (sham sorted), sorted stem cells (CD44+/CD24-/lo), and sorted cells negative for the CD44+/CD24-/lo immunophenotype (not CD44+/CD24-/lo). Error bars are standard error of the mean.