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Figure 4 | Molecular Cancer

Figure 4

From: Serotonin promotes the proliferation of serum-deprived hepatocellular carcinoma cells via upregulation of FOXO3a

Figure 4

Effect of serotonin on the expression and phosphorylation of FOXO3a in serum-deprived HCC cells. (A, B) Western blot (A) and qRT-PCR analysis (B) of FOXO3a expression in HCC cells cultured in serum free media (SFM) with or without serotonin (5-HT) for 48h; *P<0.05, t- test. (C) Western blot analysis of FOXO3a expression in Huh7 cells cultured in media containing 10% fetal bovine serum (FBS) with or without 5-HT (50 μM) for 48h; *P<0.05, t-test. (D) The subcellular localization of FOXO3a in Huh7 cells (a), HepG2 cells (b) and Hep3B cells ( c) after 48h cultured in FBS, SFM or serum free media with serotonin (SFM+5-HT). Quantification of immunofluorescence staining patterns for FOXO3a in Huh7 cells (E), HepG2 (G) cells and Hep3B cells (I); *P<0.05, t-test. ( F, H, J) Western blot analysis of total-AKT, phospho-Akt (Ser473; p-AKT), total-FOXO3a and phospho-FOXO3a (Thr32; p-FOXO3a) in serum-deprived Huh7 cells (F), HepG2 cells (H) and Hep3B cells (J) cultured in the presence or absence of serotonin (5-HT) for the indicated times.

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