Figure 1From: Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasisExpression of CX3CR1 in tumor tissues. (A) Immunohistochemical analysis of CX3CR1 or macrophage marker CD68 expression in normal colon tissues and human colon carcinoma. Histopathological differentiation grade indicates well-, moderately- and poorly-differentiated. T indicates tumor invasion depth. N indicates lymph node metastasis. M indicates distant metastasis. (×200 magnification and Scale bars = 100 μm). Quantitative analysis of CX3CR1 expression in tumor sections at different histologic grade (well, moderate and poor), different tumor stages (I-IV) and with or without recurrence 3 years after operation (n = 5-10 samples per group, with 10 fields per samples). **, P<0.01. (B) Double-color immunofluorescence analyses of macrophage and CX3CR1 expression in tumors. Tumor tissues of metastasized foci in the liver were obtained from WT and CX3CR1−/− mice at 14 days after intrasplenic SL4 cells injection. The sections were immunostained with anti-F4/80 and anti-CX3CR1 antibodies. Three independent experiments were performed. (×400 magnification and Scale bars = 50 μm).Back to article page