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Figure 3 | Molecular Cancer

Figure 3

From: Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasis

Figure 3

CX3CR1 deficiency reduces macrophages accumulation in metastasized tumor. (A) Leukocytes were gated with CD45 fluorescence vs side angle scatter (SS). Scatter plots are gated on CD45+ population cells. Macrophages (CD45+F4/80+ cells) were detected by flow from metastasized foci in the liver after intrasplenic injection of SL4 cells in WT and CX3CR1−/− mice. Data represent the means ± SEM for n = 8 mice. **, P<0.01. (B) HE staining and immunohistochemical analysis of macrophage infiltration in metastasized foci after intrasplenic injection of SL4 cells in WT and CX3CR1−/− mice. Macrophage infiltration detected by anti-Mac-2 immunostaining. (×400 magnification and Scale bars = 50 μm). Quantitative analysis of Mac-2 positive cells in metastasized foci sections. Data are mean ± SEM for n = 8 mice with 10 fields per animal. **, P<0.01.

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