Figure 3From: Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasisCX3CR1 deficiency reduces macrophages accumulation in metastasized tumor. (A) Leukocytes were gated with CD45 fluorescence vs side angle scatter (SS). Scatter plots are gated on CD45+ population cells. Macrophages (CD45+F4/80+ cells) were detected by flow from metastasized foci in the liver after intrasplenic injection of SL4 cells in WT and CX3CR1−/− mice. Data represent the means ± SEM for n = 8 mice. **, P<0.01. (B) HE staining and immunohistochemical analysis of macrophage infiltration in metastasized foci after intrasplenic injection of SL4 cells in WT and CX3CR1−/− mice. Macrophage infiltration detected by anti-Mac-2 immunostaining. (×400 magnification and Scale bars = 50 μm). Quantitative analysis of Mac-2 positive cells in metastasized foci sections. Data are mean ± SEM for n = 8 mice with 10 fields per animal. **, P<0.01.Back to article page