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Figure 6 | Molecular Cancer

Figure 6

From: The E3 ubiquitin ligases β-TrCP and FBXW7 cooperatively mediates GSK3-dependent Mcl-1 degradation induced by the Akt inhibitor API-1, resulting in apoptosis

Figure 6

Inhibition of GSK3 with SB216763 antagonizes the effects of API-1 on decreasing cell survival (A) and inducing caspase cleavage (B). A, The indicated cell lines were exposed to different concentrations of API-1 alone, SB216763 alone and the combination of API-1 and SB216763. After 3 days, cell numbers were estimated with the SRB assay. Data are means ± SDs of four replicate determinations. The numbers in the graphs are CIs for the indicated combinations. B, The given cell lines were pre-treated with 10 μM SB216763 for 30 min and then co-treated with 5 μM API-1 for 24 h and then harvested for preparation of whole-cell protein lysates and subsequent Western blotting. CF, cleaved form.

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Molecular Cancer

ISSN: 1476-4598