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Figure 7 | Molecular Cancer

Figure 7

From: RETRACTED ARTICLE: α-santalol inhibits the angiogenesis and growth of human prostate tumor growth by targeting vascular endothelial growth factor receptor 2-mediated AKT/mTOR/P70S6K signaling pathway

Figure 7

α-santalol potentiates apoptosis in HUVECs and PC-3 cells in a dose-dependent manner. (A) Apoptotic population of HUVEC and PC-3 tumor cells treated with α-santalol was detected by Hoechst 33258 staining. Apoptosis cells were observed in a large number of treated cells in both HUVEC and PC-3 cells. α-santalol induced cleavage of (B) caspase- 3 and (C) PARP. Proteins from HUVECs treated with α-santalol were analyzed by Western blotting analysis for cleaved caspase-3 and cleaved PARP. (D) Cells were grown at standard culture conditions as mentioned in Materials and methods, and treated with α- santalol, and cell lysates were prepared following cytometric bead array vendor manual. Beads containing active human caspase 3 were resolved in FL3 channel of flow cytometer followed by acquisition of sample data. BD CBA analysis software was used to analyze the data. Active caspase 3 was quantified using 4-parameter logistic curve-fit model generated standard curve from ‘human active caspase 3 lysate standard’ supplied with the kit, and presented as pg/ml cell lysate. Values are mean ± SEM (n = 6) of three independent experiments. *p < 0.05; ***p < 0.001 versus vehicle control group.

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Molecular Cancer

ISSN: 1476-4598