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Figure 4 | Molecular Cancer

Figure 4

From: Human tumor necrosis factor (TNF)-alpha-induced protein 8-like 2 suppresses hepatocellular carcinoma metastasis through inhibiting Rac1

Figure 4

TIPE2 suppressed HCC migration and invasion via inhibiting Rac1 pathway. (A) BEL-7402 and HepG2 cells that were cotransfected with Mock or TIPE2 plasmids and siNC or siRac1 were used for migration and invasion assay. (B) BEL-7402 cell was transfected with TIPE2 or mutant plasmids for 24 h, the cell lysates were prepared and immonoprecipitated with anti-Flag antibody or isotype IgG. The precipitates and cell lysates were subjected to Western blotting with anti-Flag and anti-Rac1 antibody; (C) BEL-7402 cell were transfected with Mock, wide type TIPE2 and mutant TIPE2 plasmids respectively, and cell lysates were subjected to pull down using PAK-GST protein beads. Activated Rac1 from pull down and total Rac1 in the lysates were detected by Western blot; (D) BEL-7402 and HepG2 cells that were transfected with Mock, TIPE2 and Mutant TIPE2 plasmid were used for migration and invasion assay. Data shown are representative of three independent experiments (n = 3). *P < 0.05; *** P <0.001.

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