Figure 3
From: MiR-29c is downregulated in gastric carcinomas and regulates cell proliferation by targeting RCC2
miR-29c induces growth inhibition, but not apoptosis. MKN45 cells were transfected with pre-29c or pre-Neg. (A) Activities of caspases-8, -9 and −3/7 were determined at 48 h after transfection. These data are shown as mean ± SD for three or four wells. miR-375 was used as a positive control. (B) Mitotic cells were labeled with phospho-histone H3 (Ser10) at 24 h. The proportion of positive cells was quantified by counting at least 500 cells in each of 3 or 4 wells, and is shown as a graph. (C) BrdU incorporation was determined by ELISA at 24 h. *P <0.05, **P <0.01.