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Figure 1 | Molecular Cancer

Figure 1

From: Loss of MEF2D expression inhibits differentiation and contributes to oncogenesis in rhabdomyosarcoma cells

Figure 1

MRFs and E proteins are expressed in RMS cells, but MEF2D is severely down regulated at the protein and RNA level. A. RMS cell lines express myogenin, MyoD and HEB. Extracts from each indicated cell line were western blotted and probed with antibodies against myogenin, MyoD, HEB and GAPDH. UD represents undifferentiated (proliferating) cells and D2 represents cells subjected to differentiation conditions for 2 days. B. MEF2D gene expression levels are down regulated in RMS cells. Gene expression was assayed for MEF2D from cell lines indicated as in A. C. MEF2D protein expression is down regulated in RMS cells. Protein extracts from cell lines indicated as in A. were used for western blots and probed with antibodies against MEF2D or GAPDH. D. Muscle specific genes are highly down regulated in RMS cells. mRNA expression for the indicated genes is shown for the indicated cell lines while proliferating (UD) and after differentiation for two days (D2). The number above the bars in the graphs represent the fold change between the UD and D2 samples.

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