Figure 3

Muscle specific reporters are largely inactive in RMS cells but can be stimulated by exogenous MEF2D. A. Muscle specific reporters show minimal activity in RD cells, but are completely inactive in RH30 cells. Indicated cell lines were transiently transfected with increasing concentrations of a muscle specific reporter construct. A luciferase reporter containing the leiomodin (Lmod2) promoter was used. pGL3 basic was used as the negative control. This value was set to 1, and all other values presented are relative to this value. pGL3+, which contains the constitutive CMV promoter, was used as the positive control. Error bars represent standard deviation from the mean for the three replicate samples for each data point. B. Exogenous MEF2D promotes expression of the Lmod2 reporter construct. MEF2D was transfected into RD and RH30 cells. Assay was performed as described in A.