Figure 2
From: TGM3, a candidate tumor suppressor gene, contributes to human head and neck cancer
Promoter region hypermethylation mediated the silencing of TGM3 in HNSCC. (A) Restoration of TGM3 expression by 5-Aza-dC. Three representative HNSCC cell lines were treated with 2 μM or 5 μM 5-Aza-dC for 5 days, and TGM3 transcriptional levels were determined by real-time RT-PCR. (B) Schematic representations of the location of the CpG islands in the upstream region of the TGM3 gene and the location of bisulfate-based sequencing within the CpG island. (C) Bisulfate-treated genomic DNA sequencing to detect the methylation status of the CpG islands in 5 HNSCC cell lines and 3 representative HNSCC samples compared with the methylation status in normal primary head and neck epithelial cells and paired adjacent normal tissues. White and black circles represent unmethylated and methylated CpGs, respectively.