Frequency of CD8+T cells and CD4+T cells in tumors after the treatments. Frequency of CD8+ T cells (A) and CD4+ T cells (B) in tumors was analyzed on day 1 to 9 post-treatment by FACs gated on lymphocytes. Representative staining of CD8+ T cells (C) and CD4+ T cells (D) in tumors (dark brown) was shown by IHC. Control: control group; SLC: SLC group; SLC + anti-CD25: combination therapy group. Values were presented as mean ± SD; n = 5 mice/group. * P < 0.05, ** P < 0.01 vs control or indicated comparison on day 1 to 9. Results were obtained in three independent experiments. Bar = 50 μm.