Figure 7

NF-κB p65 mediated the maturation of DCs downstream of SLC. BMDCs were pre-treated with PDTC for 1 h and then cultured with SLC or ELC for 1, 2, 4, 6, 12, 24 h. Whole cell lysates, nuclear lysates and cytosol lysates were analyzed for NF-κB p65 and phosphorylated NF-κB p65 (A), and CCR7 (B). BMDCs were pre-treated with PDTC for 1 h and then cultured with SLC for 24 h. Expression of CCR7, CD80 and CD86 was analyzed by FACs (C). Densitometer quantitation was relative to the first data set in each case (indicated by a value of 1).