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Figure 1 | Molecular Cancer

Figure 1

From: Promoting effect of neutrophils on lung tumorigenesis is mediated by CXCR2 and neutrophil elastase

Figure 1

Effect of treatment with anti-neutrophil antibody on lung inflammation and tumor promotion. (A) Total and lineage-specific leukocyte number in BALF of CC-LR mice treated or non-treated with mLy-6G Ab at the age of 14 weeks (mean ± SE; * = P ≤ 0.05 for CC-LR vs CC-LR plus mLy-6G). (B) Total and lineage-specific leukocyte number in BALF of NTHi-exposed CC-LR mice treated or non-treated with mLy-6G Ab collected 1 day after last NTHi aerosol exposure at the age of 14 weeks (mean ± SE; * = P ≤ 0.05 for CC-LR vs CC-LR plus mLy-6G with NTHi exposure). (C) Real-time Q-PCR expression analysis of arginase 1 on the RNA extracted from whole lung tissue (normalized to GAPDH expression level, mean ± SE; * = P ≤ 0.05 for CC-LR vs CC-LR with NTHi exposure; # = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus mLy-6G treatment). (D) Western blot analysis of arginase 1 on the protein extracted from whole lung tissue. (E) Lung surface tumor number after mLy-6G Ab treatment in NTHi exposed or non-exposed 14-week-old CC-LR mice. (mean ± SE; * = P ≤ 0.05 for CC-LR vs CC-LR plus mLy-6G treatment; # = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus mLy-6G treatment). (F) Histopathological appearance of lung tissue after treatment with mLy-6G Ab in NTHi exposed or non-exposed CC-LR mice. (4× magnification, scale bar = 50 mm, applicable to all panels).

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