Figure 2

Immunohistochemical analysis of lung tissue after treatment with anti-neutrophil antibody. (A) Labeling with P40 Ab to detect infiltrating neutrophils in the non-tumoral area and tumoral area of the lung in CC-LR mice with or without mLy-6G treatment in presence of NTHi (20× magnification, scale bar = 50 mm, applicable to all panels). (B) Quantitative analysis of P40 positive staining in the non-tumoral area and tumoral area of the lung in CC-LR mice with or without mLy-6G treatment in the presence of NTHi (mean ± SE; * = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus mLy-6G treatment in non-tumoral area; # = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus mLy-6G treatment in tumoral area). (C) Representative photomicrographs of Ki-67 positive cells in lung tissue of CC-LR mice with or without mLy-6G treatment in the presence or absence of NTHi (40× magnification, scale bar = 50 mm, applicable to all panels). (D) Quantitative analysis of Ki-67 positive staining in lung tissue from CC-LR mice with or without mLy-6G in the presence or absence of NTHi exposure (mean ± SE; * = P ≤ 0.05 for CC-LR vs CC-LR plus mLy-6G treatment, # = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus mLy-6G treatment). (E) Representative photomicrographs of CD31 positive cells in lung tissue of CC-LR mice with or without mLy-6G treatment in the presence or absence of NTHi (20× magnification, scale bar = 50 mm, applicable to all panels). (F) Quantitative analysis of CD31 positive staining in lung tissue of CC-LR mice with or without mLy-6G in the presence or absence of NTHi exposure (mean ± SE; * = P ≤ 0.05 for CC-LR vs CC-LR plus mLy-6G treatment).