Figure 1
TrkB overexpression is associated with global changes in miRNA expression in endometrial cancer cells. (A) Laser capture microdissection (LCM) of normal (N) endometrial cells and endometrial cancer (EC) cells. 100×. (B) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) of TrkB in endometrial cancer specimens (n = 71) and normal tissues (n = 25). TrkB expression was normalized against β-actin. Data are presented as individual samples with the line indicating the mean +/− SD. NS, P > 0.05. (C) Immunostaining scores of TrkB in normal endometrium (n = 25) and endometrial cancer specimens (n = 110). Data are presented as mean +/− SD. **, P < 0.01. (D) MicroRNA array analysis of miRNA expression in endometrial cancer cells. Hierarchical clustering of the expression values for 1347 mature miRNAs of IshikawaTrkB versus Ishikawa cells and HEC-1BshTrkB versus HEC-1B cells.