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Figure 4 | Molecular Cancer

Figure 4

From: A TrkB–STAT3–miR-204-5p regulatory circuitry controls proliferation and invasion of endometrial carcinoma cells

Figure 4

TrkB promotes while miR-204-5p suppresses the clonogenic growth, migration and invasion of endometrial cancer cells. (A) Left panel: IshikawaTrkB cells were transfected with miR-204 m or miR-204 m NC. MiR-204-5p expression was measured by Taq Man PCR assays 72 h post transfection and normalized against U6B. Data are shown as mean ± SD of at least three independent experiments. ** P < 0.01; NS, P > 0.05. Right panel: The growth curves of Ishikawa cells were plotted by MTT assays. Data are shown as mean ± SD of at least three independent experiments. * P < 0.05; NS, P > 0.05. (B) Left panel: HEC-1BshTrkB cells were transfected with miR-204i or miR-204i NC. MiR-204-5p expression was measured by Taq Man PCR assays 72 h post transfection and normalized against U6B. Data are shown as mean ± SD of at least three independent experiments. ** P < 0.01 vs. miR-204i; NS, P > 0.05. Right panel: The growth curves of HEC-1B cells were plotted by MTT assays. * P < 0.05; NS, P > 0.05. (C) IshikawaTrkB cells were transfected as in (A) and HEC-1BshTrkB cells as in (B). Colony formation was observed. Data are shown as mean ± SD of at least three independent experiments. ** P < 0.01; NS P > 0.05. (D to G) IshikawaTrkB cells were transfected with miR-204 m or miR-204 m NC and HEC-1BshTrkB cells with miR-204i or miR-204i NC. Transwell and cell invasion assays were done as described in Methods. The number of migrated cells is shown in (D and E, * P < 0.05; NS, P > 0.05). The number of invasive cells is shown in (F and G, * P < 0.05; NS, P > 0.05).

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