Table 3 Oligonucleotide sequences used in the study
Identifier | Sense primer sequences | Antisense primer sequences |
|---|---|---|
miR-204i | 5′-AGGCAUAGGAUGACAAAGGGAA-3′ | |
miR-204i NC | 5′-CAGUACUUUUGUGUAGUACAA-3′ | |
miR-204 m | 5′-UUCCCUUUGUCAUCCUAUGCCU-3′ | 5′-GCAUAGGAUGACAAAGGGAAUU-3′ |
miR-204 m NC | 5′-UUCUCCGAACGUGUCACGUTT-3′ | 5′-ACGUGACACGUUCGGAGAATT-3′ |
siTrkB | 5′-GAAUUGACGAUGGUGCAAATT-3′ | 5′-UUUGCACCAUCGUCAAUUCCA-3′ |
siTrkB-NC | 5′-UUCUCCGAACGUGUCACGUTT-3′ | 5′-ACGUGACACGUUCGGAGAATT-3′ |
siSTAT3 | 5′-GGGACCUGGUGUGAAUUAUTT-3′ | 5′-AUAAUUCACACCAGGUCCCTT-3′ |
TrkB-3′UTR-WT | 5′-GCTCTAGATTTTGGCATTATCTCTTTCTCT-3′ | 5′-GCTCTAGATTGTTCCTCCCATATTGCG-3′ |
TrkB-3′UTR-MT | 5′-GACAACAAATATTTCACTTAA-3′ | 5′-CTTTGATGTGGATGAAAAACGGTTACAAC-3′ |
GV219 STAT3 vector | 5′-CGCAAATGGGCGGTAGGCGTG-3′ | 5′-CCCACTGTCCTTTCCTAATAA-3′ |
GV254-hsa-miR-204 | 5′-AGCTGTACAAGTAAGCCTGATCATGTACCCATAGG-3′ | 5′-GGGAGAGGGGCTTAGCTTATGGGACAGTTATGGGC-3′ |