G3BP1 depletion increases PMP22 mRNA expression. PMP22 mRNA levels were analyzed with qPCR in MCF-7 (A), MDA-MB-231 (C), MDA-MB-468 (D) or BT549 (E) cells treated with 40 nM siRNA targeting G3BP1 and/or G3BP2 for 72 hours. (B) MCF-7 cells were treated with three separate G3BP1 siRNAs and PMP22 mRNA levels were analyzed. Data (mean ± SEM, n = 3) were normalized to three house-keeping genes SDHA, YWHAZ and UBC and related to levels in cells transfected with control siRNA. * < 0.05; ** < 0.01; *** < 0.001 according to ANOVA followed by Duncan’s multiple range test.