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Figure 2 | Molecular Cancer

Figure 2

From: DKK1 promotes hepatocellular carcinoma cell migration and invasion through β-catenin/MMP7 signaling pathway

Figure 2

DKK1 up-regulation or down-regulation did not influence HCC cell proliferation. The expression of DKK1 mRNA (A, B) and protein (C, D) were detected in HepG2 cells without transfection (lane 1) or transfected with Vector (lane 2) or DKK1 (lane 3), or Bel7402 cells without transfection (lane 1) or tumor cells transfected with shControl (lane 2) or shDKK1 (lane 3) using RT-PCR and western blotting. GAPDH and β-actin were used as loading controls. E, F: MTT assay showed that up-regulation or down-regulation of DKK1 did not influence the proliferation rates in HepG2 or Bel-7402 cells. G, H: enforced expression or suppression of DKK1 did not influence the colony formation in both HepG2 and Bel7402 HCC cells. HepG2 cells without transfection or transfected with Vector or DKK1 (G), and Bel7402 cells that without transfection or transfected with shControl or shDKK1 (H) were seed into 6-well plates and incubated in RPMI1640 supplemented with 10% FCS, the medium was changed every three days. 15 days later, colonies were fixed and stained with 0.1% crystal violet in 20% methanol for 20 min. Colonies composed of more than 50 cells were counted in each well. The data are represented as means ± SEM from 4 replicates in each of the 3 independent experiments.

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