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Figure 3 | Molecular Cancer

Figure 3

From: DKK1 promotes hepatocellular carcinoma cell migration and invasion through β-catenin/MMP7 signaling pathway

Figure 3

DKK1 was involved in HCC cell migration and invasion. A: Wound healing assay indicated that DKK1 up-regulation promoted HepG2 cell migration. B, C: Up-regulation of DKK1 significantly enhanced HepG2 cell migration and invasion. HepG2 cells without transfection or transfected with Vector or DKK1 were added to the transwell chamber coated without or with Matrigel for 24 h. Representative images and the number of migrated or invaded cells are shown. Analysis was performed in three separate experiments (n = 3). **, p <0.01. D: Wound healing analysis of migration in Bel7402 cells. E, F: Depleting of DKK1 attenuated the migration and invasion in Bel7402 cells. Bel7402 cells without transfection or transfected with shControl or shDKK1 cells were seeded into the transwell chamber coated without or with Matrigel and incubated in RPMI1640 containing 10% FCS for 24 h. and then fixed and stained with crystal violet for 20 min. Representative images and the number of migrated or invaded cells are shown. Results are presented as mean ± SEM (n = 3). **, p <0.01. G, H: Analysis of DKK1 expression in primary (n = 30) and metastatic (n = 41) HCC tissues by IHC. Images were captured at × 200 for DKK1 staining. Scale bar = 50 μm. The data are represented as means ± SEM. **, p <0.01.

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