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Table 2 Apoptosis detection by annexin V binding/PI uptake assay in SiHa parental and SiHa CDV

From: Reduced tumorigenicity and pathogenicity of cervical carcinoma SiHa cells selected for resistance to cidofovir

 

SiHa parental

SiHa CDV

 

Untreated

CDV 158.7 μM

CDV 63.5 μM

PMEG 6.5 μM

Untreated

CDV 158.7 μM

CDV 63.5 μM

PMEG 6.5 μM

Early apoptotic

1.2 ± 0.9

22.1 ± 0.6

8.4 ± 1.6

64.2 ± 1.2

4.5 ± 2.0

2.3 ± 0.1

2.4 ± 0.2

30.6 ± 4.3

Late apoptotic

0.6 ± 0.2

5.0 ± 5.6

2.7 ± 2.5

6.0 ± 4.2

0.9 ± 0.4

0.6 ± 0.1

0.6 ± 0.1

3.9 ± 0.7

Viable

97.2 ± 2.2

72.2 ± 6.6

88.0 ± 3.9

29.4 ± 3.0

94.5 ± 2.2

96.3 ± 0.5

96.5 ± 0.3

64.9 ± 1.2

Necrotic

0.4 ± 0.3

0.7 ± 0.3

0.9 ± 0.2

0.4 ± 0.1

0.2 ± 0.1

0.8 ± 0.6

0.5 ± 0.2

0.7 ± 0.3

  1. Cells were seeded in 6-well microtiter plates at a density of 5 × 104 cells/well and allowed to proliferate for 24 h. Culture medium was removed and medium containing the compounds was added. At 7 days post-treatment, cells were harvested with EDTA/PBS (760 mg/L). After rinsing the cells with PBS, the cell pellets were simultaneously stained with annexin-FITC and PI using the annexin-V-FITC staining kit (BD Biosciences, San Jose CA) and analyzed by flow cytometry. Dual fluorescence dot plots were obtained following combined annexin V-FITC and PI staining. Data represent the percentages in each quadrant of the dot plots and are the mean ± SD of at least two stainings. Viable (annexin V-/PI-) early apoptotic (annexin V+/PI-), necrotic (annexin V-/PI+), and late apoptotic (annexin V+/PI+) cells.
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Molecular Cancer

ISSN: 1476-4598