Acetylation of p53 and interaction with CBP/p300 and Id4. A. p53, immuno-precipitated from cell lines was blotted with antibodies against acetylated lysine (global), p53 acetylated at either K373 (Ac-373) or K320 (Ac-320), CBP/ p300 and Id4. B. The total protein lysate from cell lines as indicated was immuno-precipitated (IP) with Id4 antibody. The immuno-precipitated lysate was then immuno-blotted with p53 antibody (IB: p53). Representative data is shown.