Effects of the sorafenib treatment on HCC cell lines. The HepG2, HA22T/VGH, SKHep1C3 and HuH6 cell lines were treated with sorafenib (5, 10, 15 μM concentration) for 3 days (24, 48 and 72 h). The proliferation was inhibited in a dose-dependent manner in all the cell lines considered (A-D). The most responsive HCC cell line was HepG2 (the highest percentage of the inhibition of proliferation was 72% with 15 μM of sorafenib at T = 72 h) (A). Statistical significance was determined by one way ANOVA with Bonferroni correction *p < 0.05; ***p < 0.001.