Figure 2

Ectopic expression of miR-124 suppresses breast cancer cell proliferation, migration and invasion. A, miR-124 was re-expressed in MDA-MB-231 and T47D cells by transfection of miR-124 mimics. Forty-eight hours later, the miR-124 expression levels were tested by quantitative RT-PCR and normalized to 5S rRNA. B, Analysis of the effect of miR-124 on the proliferation of MDA-MB-231 and T47D cells by MTT assay. C, Cell cycle arrest effect of miR-124 on MDA-MB-231 and T47D cells monitored with flow cytometric. D, Colony formation assay demonstrated that the effect of miR-124 on the cell growth of MDA-MB-231 and T47D cells. E, Analysis the effect of miR-124 on the migration of MDA-MB-231 and T47D cells by wound-healing assay. F, A Matrigel invasion chamber was utilized to analyze the effect of miR-124 on the cell invasion of MDA-MB-231 and T47D cells. The data represent the mean values of three independent experiments. *, P < 0.05 with paired t- test.