The effect of the oligonucleotides on proliferation and apoptosis of CRC cells. (A) Colony formation assay performed for SW-480 cells. The number of colonies on the entire plate was counted. (B-D) Induced apoptosis in CRC cells. After 48 h of incubation in the presence of controls or siCXCR4 and following 24-h exposure to cisplatin, all of the cells were stained with Annexin V–FITC and propidium iodide (PI) followed by flow cytometric analysis. Data are expressed as the mean ± SD of three independent experiments. (B) miR-133b mimic strengthened apoptosis in CRC cells. (C) The miR-133b inhibitor suppressed apoptosis in CRC cells. (D) siCXCR4 increased apoptosis in CRC cells.