Figure 4From: miR-133b, a muscle-specific microRNA, is a novel prognostic marker that participates in the progression of human colorectal cancer via regulation of CXCR4 expressionThe effect of the oligonucleotides on proliferation and apoptosis of CRC cells. (A) Colony formation assay performed for SW-480 cells. The number of colonies on the entire plate was counted. (B-D) Induced apoptosis in CRC cells. After 48 h of incubation in the presence of controls or siCXCR4 and following 24-h exposure to cisplatin, all of the cells were stained with Annexin V–FITC and propidium iodide (PI) followed by flow cytometric analysis. Data are expressed as the mean ± SD of three independent experiments. (B) miR-133b mimic strengthened apoptosis in CRC cells. (C) The miR-133b inhibitor suppressed apoptosis in CRC cells. (D) siCXCR4 increased apoptosis in CRC cells.Back to article page