Signalling molecules in CD133lowand CD133highcells. (A) Immunochemical analysis with the indicated antibodies of total lysates from CD133low and CD133high cells. Tubulin was blotted as a control for the equivalence of loaded protein. (B) Western blot analysis of PLC-β2 of immunoprecipitates with an anti-PLC-β2 antibody from CD133low and CD133high cells. Lysates from the same cells were analyzed for β-tubulin content, as internal control of processed proteins. The data are representative of three separate experiments.