PLC-β2 and CD133 expression. (A) Western blot analysis with the indicated antibodies of CD133low and CD133high cells transfected with a construct expressing EGFP-tagged human PLC-β2 (EGFP-PLC-β2). (B) Dynamic monitoring of invasion through Matrigel using the xCELLigence system of transfected cells. (C) Cytofluorimetrical analysis of surface expression of CD133 by direct staining with PE-conjugated 293C3 antibody. (D) Cytofluorimetrical analysis of intracellular CD133 with PE-conjugated 293C3 antibody of transfected CD133high cells. Only EGFP-expressing cells were analyzed. The asterisks indicate statistically significant differences (P < 0.05). Error bars indicate ± SD. All the data are representative of three separate experiments.