PLC-β2 and invasiveness of CD133lowcells. (A) Immunochemical analysis of immunoprecipitates with the anti-PLC-β2 antibody from CD133low cells transfected with siRNAs specific for PLC-β2 (PLC-β2 siRNAs). A non-silencing scramble siRNAs was used as a control (ctrl siRNAs). Lysates from the same cells were analyzed for β-tubulin content, as internal control of processed proteins. (B) Cytofluorimetrical analysis of surface expression of CD133 by direct staining with PE-conjugated 293C3 antibody and (C) dynamic monitoring of invasion through Matrigel using the xCELLigence system of transfected cells. Error bars indicate ± SD. All the data are representative of three separate experiments.