CD133 and PLC-β2 expression in MDA-MB-468 cells. (A) CD133 surface expression evaluated in MDA-MB-468 cells by means of flow cytometry after staining with PE-conjugated 293C3 antibody and presented on a biparametric dot plot. The quadrants are gated to separate positive populations. The percentage of cells expressing high levels of CD133 is indicated at the upper right of each panel, together with their mean fluorescence intensity (MFI). (B) Western blot analysis with the indicated antibodies of MDA-MB-468 cells transfected with a construct expressing EGFP-tagged human PLC-β2 (EGFP-PLC-β2). (C) Cytofluorimetrical analysis of surface expression of CD133 by direct staining with PE-conjugated 293C3 antibody of transfected cells. Only EGFP-expressing cells were analyzed. The asterisk indicates statistically significant differences (P < 0.05). (D) Dynamic monitoring of invasion through Matrigel using the xCELLigence system of transfected cells. Error bars indicate ± SD. All the data are representative of three separate experiments.