Activation of cellular immunity and marked inhibition of liver cancer in a mouse model following gene therapy and tumor expression of GM-SCF, IL-21, and Rae-1

Table 1 Showing the primer sequences used to amplify the genes for GM-CSF, GFP, Rae-1, and IL-21

Genes	Primers
GM-CSF-XhoI-F	CAGATCTCGAG GCCACCATGTGGCTGCAGAATTTACTTTTC
GM-CSF-EcoRI-R	GTCAGAATTC CCATTTTTGGCCTGGTTTTTTG
GFP-EcoRI-F	GTCAGAATTC ATGGTGAGCAAGGGCGAG
GFP-MluI-R	ACTTAACGCGT TTACTTGTACAGCTCGTCCATGC
Rae-1-Xba1-F	GTACATTCTAGA GCCACCATGAGTCTGTTTGGATCAACCTCT
Rae-1-Sal1-R	GAATGTCGAC GTATTTCTTATTCCTTGGCTTTAGCT
IL-21-Sal1-F	GTCAGTCGAC GCCACCATGGAGAGGACCCTTGTCTGTC
IL-21-Not1-R	AATTGCGGCCGC CTAGGAGAGATGCTGATGAATCATC

Note: Italics indicates the enzyme cleavage sites for XhoI and EcoRI, EcoRI and MluI, Xba1and Sal1, and Sal1and Not1.

ISSN: 1476-4598