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Figure 3 | Molecular Cancer

Figure 3

From: Reduced annexin A6 expression promotes the degradation of activated epidermal growth factor receptor and sensitizes invasive breast cancer cells to EGFR-targeted tyrosine kinase inhibitors

Figure 3

Down regulation of AnxA6 in invasive breast cancer cells enhances the degradation of cell surface-associated EGFR. (A) AnxA6 depleted (BT-A6sh5) and control (BT-EV) cells were treated with EGF for 0–90 min and the cell surface-associated proteins were biotinylated as described in “materials and methods”. The biotinylated proteins were isolated from whole cell lysates using Streptavidin-agarose beads and analyzed by Western blotting using antibodies to activated and total EGFR. Ponceau S staining of the blots was used as loading control. (B and C) Densitometric analysis of residual cell surface-associated activated EGFR (B) and cell surface-associated total EGFR (C). Points represent residual biotin-labeled cell surface-associated EGFR in the control (solid lines with closed circles) and AnxA6-depleted cells (broken lines with open circles) at the indicated times from a representative experiment. IP = immunoprecipitation; WB = western blot.

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Molecular Cancer

ISSN: 1476-4598