Apoptotic cell-death measured by PI staining in breast CSCs. Cells were co-treated with Rott (2 μM) and Baf (10 nM), 3-MA (10 mM) or CHX (10 μg/ml). (a) Rott (2 μM) and Baf (10 nM), (b) Rott (2 μM) and CHX (10 μg/ml), and (c) Rott (2 μM) and 3-MA (10 mM) in complete stem cell culture medium for 48 h. Apoptosis was measured by PI staining followed by flow cytometry. Data are the means of triplicate experiments. (d) Kinetic evaluation of cells underwent apoptotic cell death in breast CSCs. Data are reported as the mean ± standard error (SE) of percentage of cells. n = 5, *P < 0.05 when compared with Rott treated in an identical manner.