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Figure 1 | Molecular Cancer

Figure 1

From: DNA-dependent protein kinase catalytic subunit (DNA-PKcs)-SIN1 association mediates ultraviolet B (UVB)-induced Akt Ser-473 phosphorylation and skin cell survival

Figure 1

UVB activates Akt, mTORC1 and mTORC2 signalings in primary skin keratinocytes. Primary cultured human skin keratinocytes were exposed to UVB radiation (UV, 30 mJ/cm2) and cultured in DMEM for indicated time (0′, 15′, 30′, 1 hr and 2 hr) (A, C and E), or irradiated with indicated concentration of UVB (0, 5, 15 and 30 mJ/cm2), and cultured for 1 hr (B, D, F), activation of Akt, mTORC1 and mTORC2 in these cells was tested by immuno-blots using phosphorylation antibodies described. Non-phosphorylated kinases were also tested as loading controls. Phosphorylation (p) of Akt (Ser-473), mTOR (Ser 2448) and Rictor (Ser 792) was quantified. The values in the figures were expressed as the means ± standard deviation (SD) (Same for the following figures). All experiments were repeated three times and similar results were obtained (Same for the following figures). Statistical significance was analyzed by ANOVA (Same for the following figures).*p < 0.05 vs. 0′.

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Molecular Cancer

ISSN: 1476-4598