Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: Epigenetic inactivation of miR-9 family microRNAs in chronic lymphocytic leukemia - implications on constitutive activation of NFκB pathway

Figure 1

Methylation of miR-9-3 and miR-9-1 in controls. A, U- and M-MSP of miR-9-3 and miR-9-1 showed that the positive control (PC) was completely methylated while all 8 normal controls (N1-N8) were completely unmethylated. B, Sequence analysis of the miR-9-3 and miR-9-1 M-MSP product from bisulfite-treated positive control DNA respectively showed that the cytosine (C) residues of CpG dinucleotides were methylated and remained unchanged, whereas all the other C residues were unmethylated and were converted to thymidine [T], suggesting complete bisulfite conversion and specificity of MSP. MW: Marker; B: Reagent blank; N1 to N3: CD19 sorted B cells of the peripheral blood from healthy donors; N4 to N5: peripheral blood buffy coat from healthy donors; N6 to N8: normal bone marrow control; PC: positive control with methylated DNA.

Back to article page

Molecular Cancer

ISSN: 1476-4598